The Application of Ultrasound and Enzymes Could Be Promising Tools for Recovering Polyphenols during the Aging on Lees Process in Red Winemaking
Open Access
Abstract
The final concentration of phenolic compounds in wines is usually lower than what might be expected, given their concentration in grapes. This is in part due to the interactions between cell walls from grapes and yeast with phenolics during red winemaking. Most of these aggregates are insoluble and end up precipitating, forming part of the lees. The objective of this study is to determine the capacity of ultrasounds and/or enzymes treatments (β-glucanase and a pectolytic enzyme) to release the anthocyanins and tannins adsorbed in the lees. The ultrasound (US) applied for 120 min slightly favored the extraction of anthocyanins and doubled tannin extraction. Shorter sonication times did not show any positive effect. The combination of β-glucanase and pectolytic enzyme was always more effective in the liberation of anthocyanins (both no-acylated and acylated anthocyanins) and tannins than the enzymes acting separately. The combination of US (120 min), β-glucanase and pectolytic enzyme showed an additive effect, increasing the extraction of phenolic compounds with respect to the individual treatments and also releasing a large quantity of low molecular weight polysaccharides, compounds of enological importance. These results of this study could be of enological interest, facilitating and accelerating the aging on lees process, through the liberation of polysaccharides and the recovery of the phenolic compounds lost during vinification.Keywords
Funding Information
- MInisterio de Economia y Competitividad, ,Gobierno de España (AGL2015-65974-R, and European FEDER funds)
This publication has 46 references indexed in Scilit:
- Evaluation of the Combined Effects of Enzymatic Treatment and Aging on Lees on the Aroma of Wine fromBombino biancoGrapesJournal of Agricultural and Food Chemistry, 2008
- Evolution of red wine anthocyanins during malolactic fermentation, postfermentative treatments and ageing with leesFood Chemistry, 2008
- Conventional and enzyme-assisted autolysis during ageing over lees in red wines: Influence on the release of polysaccharides from yeast cell walls and on wine monomeric anthocyanin contentFood Chemistry, 2007
- Interactions between Yeast Lees and Wine Polyphenols during Simulation of Wine Aging: II. Analysis of Desorbed Polyphenol Compounds from Yeast LeesJournal of Agricultural and Food Chemistry, 2006
- Adsorption of Anthocyanins by Yeast Cell Walls during the Fermentation of Red WinesJournal of Agricultural and Food Chemistry, 2003
- Maintenance and Protection of Yeast Morphology by Contact with Wine Polyphenols During Simulation of Wine Aging on LeesJournal of Food Science, 2003
- Effect of oxygenation on polyphenol changes occurring in the course of wine-makingAnalytica Chimica Acta, 2002
- Release of nitrogen compounds to the extracellular medium by three strains of Saccharomyces cerevisiae during induced autolysis in a model wine systemInternational Journal of Food Microbiology, 2001
- Isolation, Identification, and Characterization of New Color-Stable Anthocyanins Occurring in Some Red WinesJournal of Agricultural and Food Chemistry, 1997
- Distribution of chitin in the yeast cell wall. An ultrastructural and chemical study.Journal of Cell Biology, 1980