Design of a Selective Metal Ion Switch for Self-Assembly of Peptide-Based Fibrils
- 8 December 2007
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 130 (1) , 49-51
Abstract
The self-assembling peptide TZ1H, a structural variant of the trimeric isoleucine zipper GCN4-pII, contains histidine residues at core d-positions of alternate heptads that define three trigonal coordination sites within the coiled-coil trimer. Circular dichroism spectropolarimetry indicated that peptide TZ1H undergoes a random coil to α-helical conformational change upon binding of 1 equiv of silver(I) ion, but not zinc(II), copper(II), or nickel(II) ions. Isothermal titration calorimetry provided evidence for a single binding-site model in which each peptide contributes one net silver(I) coordination site, in agreement with the proposed structural model. Transmission electron microscopy revealed that TZ1H self-assembles into long aspect ratio helical fibers in the presence of silver(I) ion. These results demonstrate that the rational design of selective metal ion binding sites within de novo designed peptides represents a promising approach to the controlled fabrication of nanoscale, self-assembled materials.Keywords
This publication has 26 references indexed in Scilit:
- Incorporating Electron-Transfer Functionality into Synthetic Metalloproteins from the Bottom-upInorganic Chemistry, 2006
- Rational Design of a Reversible pH-Responsive Switch for Peptide Self-AssemblyJournal of the American Chemical Society, 2006
- Computational Design of a Single Amino Acid Sequence that Can Switch between Two Distinct Protein FoldsJournal of the American Chemical Society, 2006
- Metal-induced folding of a designed metalloproteinJournal of Inorganic Biochemistry, 2004
- Binding of Cu(II) or Zn(II) in a de novo designed triple‐stranded α‐helical coiled‐coil toward a prototype for a metalloenzymeChemical Biology & Drug Design, 2004
- Engineering the morphology of a self-assembling protein fibreNature Materials, 2003
- De novo design of fibrils made of short α-helical coiled coil peptidesChemistry & Biology, 2001
- Crystal structure of GCN4-pIQI, a trimeric coiled coil with buried polar residuesJournal of Molecular Biology, 1998
- An Infinite 2D Polyrotaxane Network in Ag2(bix)3(NO3)2 (bix = 1,4-Bis(imidazol-1-ylmethyl)benzene)Journal of the American Chemical Society, 1997
- New aspects of the reaction of silver(I) cations with the ethylenediaminetetraacetate ionTalanta, 1995