Practical screening of purified cellobiohydrolases and endoglucanases with α-cellulose and specification of hydrodynamics
Open Access
- 18 August 2010
- journal article
- Published by Springer Nature in Biotechnology for Biofuels
- Vol. 3 (1) , 18
Abstract
It is important to generate biofuels and society must be weaned from its dependency on fossil fuels. In order to produce biofuels, lignocellulose is pretreated and the resulting cellulose is hydrolyzed by cellulases such as cellobiohydrolases (CBH) and endoglucanases (EG). Until now, the biofuel industry has usually applied impractical celluloses to screen for cellulases capable of degrading naturally occurring, insoluble cellulose. This study investigates how these cellulases adsorb and hydrolyze insoluble α-cellulose − considered to be a more practical substrate which mimics the alkaline-pretreated biomass used in biorefineries. Moreover, this study investigates how hydrodynamics affects cellulase adsorption and activity onto α-cellulose.Keywords
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