Comparison of anti‑inflammatory effects of Mecasin and its constituents on lipopolysaccharide‑stimulated BV2 cells
Open Access
- 8 April 2021
- journal article
- research article
- Published by Spandidos Publications in Experimental and Therapeutic Medicine
- Vol. 21 (6) , 1-7
Abstract
Mecasin, a traditional medicine, contains nine herbal constituents: Curcuma longa, Salvia miltio rhiza, Gastrodia elata, Chaenomeles sinensis, Polygala tenuifolia, Paeonia japonica, Glycyrrhiza uralensis, Atractylodes japonica and processed Aconitum carmichaeli. Several biological effects of mecasin have been described both in vivo and in vitro. Previous studies have demonstrated that mecasin has anti‑inflammatory effects. The purpose of the present study was to determine anti‑inflammatory effects of mecasin and its natural product constituents on lipopolysaccharide (LPS)‑stimulated BV2 cells by measuring nitrite and nitric oxide contents. Nitrite production levels in LPS‑stimulated BV2 cells incubated with mecasin and each individual constituent of mecasin were measured. The results suggested that C. longa, P. tenuifolia and P. japonica inhibited nitrite production in a pattern similar to that of mecasin. The effect of mecasin was likely a result of synergistic effects of its natural herb constituents.Keywords
This publication has 18 references indexed in Scilit:
- A 4-week Repeated dose Oral Toxicity Study of Mecasin in Sprague-Dawley Rats to Determine the Appropriate Doses for a 13-week, Repeated Toxicity TestJournal of Pharmacopuncture, 2015
- A Study on the Oral Toxicity of Mecasin in RatsJournal of Pharmacopuncture, 2014
- Innate immune responses regulate morphogenesis and degeneration: roles of Toll-like receptors and Sarm1 in neuronsNeuroscience Bulletin, 2014
- Intravital correlated microscopy reveals differential macrophage and microglial dynamics during resolution of neuroinflammationDisease Models & Mechanisms, 2014
- KCHO‐1, a Novel Antineuroinflammatory Agent, Inhibits Lipopolysaccharide‐Induced Neuroinflammatory Responses through Nrf2‐Mediated Heme Oxygenase‐1 Expression in Mouse BV2 Microglia CellsEvidence-Based Complementary and Alternative Medicine, 2014
- Inflammation in neurodegenerative diseases – an updateImmunology, 2013
- Ganoderma lucidum ethanol extract inhibits the inflammatory response by suppressing the NF-κB and toll-like receptor pathways in lipopolysaccharide-stimulated BV2 microglial cellsExperimental and Therapeutic Medicine, 2013
- The Multiple Roles of the Innate Immune System in the Regulation of Apoptosis and Inflammation in the BrainJournal of Neuropathology & Experimental Neurology, 2009
- Orthogonal array design for optimizing extraction efficiency of active constituents from Jakyak-Gamcho Decoction, the complex formula of herbal medicines, Paeoniae Radix and Glycyrrhizae RadixJournal of Ethnopharmacology, 2007
- Targeting IL-1 in inflammatory disease: new opportunities for therapeutic interventionNature Reviews Drug Discovery, 2004