Screening of Anthocyanins and Anthocyanin-Derived Pigments in Red Wine Grape Pomace Using LC-DAD/MS and MALDI-TOF Techniques

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Abstract
Two phenolic extracts were made from a red wine grape pomace (GP) and fractionated first by sequential liquid–liquid extraction with organic solvents. The aqueous fraction was fractionated by low-pressure chromatography on Toyopearl HW-40 gel and on C18. Different fractions were obtained by sequential elution with aqueous/organic solvents, and then analyzed by liquid chromatography and mass spectrometry (LC-DAD/MS and MALDI-TOF). Over 50 anthocyanin-based pigments were detected by LC-DAD/MS in GP, mainly pyranoanthocyanins including A- and B-type vitisins and methylpyranoanthocyanins. The presence of oligomeric malvidin-3-O-coumaroylglucoside-based anthocyanins was also detected in GP using both LC-DAD/MS and MALDI-TOF.
Funding Information
  • Secretaría de Ciencia, Humanidades, Tecnología e Innovación (204199)
  • European Social Fund Plus
  • Fundação para a Ciência e a Tecnologia (SFRH/BD/70053/2010, SFRH/BPD/65400/2009)
  • European Regional Development Fund

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