Optimization of glutamate concentration and pH for H2 production from volatile fatty acids by Rhodopseudomonas capsulata

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Abstract
Aims: This study attempted to employ response surface methodology (RSM) to evaluate the effects of glutamate concentration and pH on H2 production from volatile fatty acids by Rhodopseudomonas capsulata. Methods and Results: A mixture of acetate, propionate and butyrate was used as a carbon source for the H2 production by R. capsulata. The H2 yield and H2 production rate were strongly affected by the glutamate concentration, pH and their interaction. The predicted maximum H2 yield of 0·534 was obtained when glutamate concentration and pH were 6·56 mmol l−1 and 7·29 respectively. On the contrary, the maximum H2 production rate of 18·72 ml l−1 h−1 was achieved at a glutamate concentration of 7·01 mmol l−1 and pH 7·31. Conclusions: Taking H2 yield and H2 production rate together into account, a glutamate concentration of 6·56–7·01 mmol l−1 and pH of 7·29–7·31 should be selected for H2 production from a mixture of acetate, propionate and butyrate by R. capsulata. Significance and Impact of the Study: The RSM was a useful tool for maximizing H2 production by photosynthetic bacteria (PSB).

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