Proteomic Analysis of the Carbonate Insoluble Outer Membrane Fraction of the Soft-Rot Pathogen Dickeya dadantii (syn. Erwinia chrysanthemi) Strain 3937
- 7 December 2006
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Proteome Research
- Vol. 6 (1) , 62-69
Abstract
We present results of the first comprehensive proteomic analysis of the outer membrane of the bacterial phytopathogen Dickeya dadantii strain 3937 and its response to virulence-contributing factors such as host plant extract, acidic stress, and iron starvation. We analyzed the carbonate-insoluble membrane fractions, which are highly enriched for outer membrane proteins, using two-dimensional electrophoresis and identified the proteins by MALDI-TOF MS. Forty unique proteins were identified, some of which were differentially expressed under the above conditions. Keywords: Dickeya dadantii • outer membrane • proteome • virulence factors • plant extract • acidic stress • iron starvation • post-translational modificationsKeywords
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