Expression ofAnaplasma marginaleMajor Surface Protein 2 Variants in Persistently Infected Ticks
- 1 August 2001
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 69 (8) , 5151-6
Abstract
Anaplasma marginale, an intraerythrocytic ehrlichial pathogen of cattle, establishes persistent infections in both vertebrate (cattle) and invertebrate (tick) hosts. The ability ofA. marginaleto persist in cattle has been shown to be due, in part, to major surface protein 2 (MSP2) variants which are hypothesized to emerge in response to the bovine immune response. MSP2 antigenic variation has not been studied in persistently infected ticks. In this study we analyzed MSP2 inA. marginalepopulations from the salivary glands of maleDermacentor variabilispersistently infected withA. marginaleafter feeding successively on one susceptible bovine and three sheep. New MSP2 variants appeared in eachA. marginalepopulation, and sequence alignment of the MSP2 variants revealed multiple amino acid substitutions, insertions, and deletions. These results suggest that selection pressure on MSP2 occurred in tick salivary glands independent of the bovine immune response.Keywords
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