Determination of the Functional Groups in Algae Parachlorella Kessleri by Potentiometric Titrations

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Abstract
The acidic functional groups of the cell wall of native algae Parachlorella kessleri were evaluated by potentiometric titrations. The Gran´s method was applied to determination of the total, strong, weak and very weak acidities. The total organic acidity obtained for biomass was 3.93 mmol g-1, the largest content belonged to the strong acidic groups (2.13 mmol g-1) together with the weak acidic carboxylic groups (1.28 mmol g-1). Very weak acidities represented by the amine groups (0.52 mmol g-1) did not exceed 14% and they formed the lowest numerous part of all acidic functional groups.