Nested U-Net-Based GAN Model for Super-Resolution of Stained Light Microscopy Images

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Abstract
The purpose of this study was to propose a deep learning-based model for the super-resolution reconstruction of stained light microscopy images. To achieve this, perceptual loss was applied to the generator to reflect multichannel signal intensity, distribution, and structural similarity. A nested U-Net architecture was employed to address the representational limitations of the conventional U-Net. For quantitative evaluation, the peak signal-to-noise ratio (PSNR), structural similarity index (SSIM), and correlation coefficient (CC) were calculated. In addition, intensity profile analysis was performed to assess the model’s ability to restore the boundary signals more precisely. The experimental results demonstrated that the proposed model outperformed both the signal and structural restoration compared to single U-Net and U-Net-based generative adversarial network (GAN) models. Consequently, the PSNR, SSIM, and CC values demonstrated relative improvements of approximately 1.017, 1.023, and 1.010 times, respectively, compared to the input images. In particular, the intensity profile analysis confirmed the effectiveness of the nested U-Net-based generator in restoring cellular boundaries and structures in the stained microscopy images. In conclusion, the proposed model effectively enhanced the resolution of stained light microscopy images acquired in a multichannel format.

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