Inhibition of erythrocyte ATPase activity by aclacinomycin and reverse effects of ascorbate on ATPase activity
Authors:
- 1 December 1983
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 39 (12) , 1362-1364
Abstract
We studied the effect of aclacinomycin on human erythrocyte membrane enzymes. Aclacinomycin inhibited ATPase, including Na−K-dependent ATPase, ouabain insensitive ATPase and Ca-ATPase. However acetylcholinesterase was not inhibited by aclacinomycin. The ATPase activities were not inhibited by aclacinomycin if ascorbate was added to the incubation mixture. However other reducing agents, α-tocopherol, superoxide dismutase and catalase had no effect on ATPase activity. Ascorbate may protect membrane proteins and lipids from peroxidate damage.Keywords
This publication has 9 references indexed in Scilit:
- The Effects of Adriamycin (Doxorubicin HCL) on Human Red Blood CellsHemoglobin, 1980
- Clinical study of aclacinomycin A.1979
- Adriamycin and daunomycin generate reactive oxygen compounds in erythrocytesBlood, 1978
- ADRIAMYCIN AND DAUNOMYCIN GENERATE REACTIVE OXYGEN COMPOUNDS IN ERYTHROCYTES1978
- Generation of free radicals and lipid peroxidation by redox cycling of adriamycin and daunomycinBiochemical and Biophysical Research Communications, 1977
- International Committee for Standardization in Haematology: Recommended Methods for Red‐Cell Enzyme Analysis*British Journal of Haematology, 1977
- Separation of two adenosine triphosphatases from erythrocyte membraneBiochemical and Biophysical Research Communications, 1963
- The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytesArchives of Biochemistry and Biophysics, 1963
- Determination of Inorganic PhosphateAnalytical Chemistry, 1949