Immunoassay method for the determination of immunoglobulin G using bacterial magnetic particles

Authors:
  • KHKohji. Hashimoto
    Kohji. Hashimoto
  • TMTadashi. Matsunaga
    Tadashi. Matsunaga
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Abstract
We have developed a novel immunoassay method using bacterial magnetic particles for the determination of immunoglobulin G (IgG). Fluorescein isothiocyanate (FITC) conjugated anti IgG-bacterial magnetic particles were prepared. The fluorescence quenching caused by agglutination of FITC-anti IgG antibody-bacterial magnetic particle conjugates was measured by using a fluorescence spectrophotometer. The aggregates based on specific immunoreaction were separated by a gelatin solution. The aggregation of bacterial magnetic particle conjugates was enhanced by application of a magnetic field. The relative fluorescence intensity correlated linearly with a concentration of IgG in the range 0.5-100 ng/mL.