Activity and Function in Human Cells of the Evolutionary Conserved Exonuclease Polynucleotide Phosphorylase
Open Access
- 31 January 2022
- journal article
- review article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 23 (3) , 1652
Abstract
Polynucleotide phosphorylase (PNPase) is a phosphorolytic RNA exonuclease highly conserved throughout evolution. Human PNPase (hPNPase) is located in mitochondria and is essential for mitochondrial function and homeostasis. Not surprisingly, mutations in the PNPT1 gene, encoding hPNPase, cause serious diseases. hPNPase has been implicated in a plethora of processes taking place in different cell compartments and involving other proteins, some of which physically interact with hPNPase. This paper reviews hPNPase RNA binding and catalytic activity in relation with the protein structure and in comparison, with the activity of bacterial PNPases. The functions ascribed to hPNPase in different cell compartments are discussed, highlighting the gaps that still need to be filled to understand the physiological role of this ancient protein in human cells.Keywords
This publication has 133 references indexed in Scilit:
- A Mutation in PNPT1, Encoding Mitochondrial-RNA-Import Protein PNPase, Causes Hereditary Hearing LossThe American Journal of Human Genetics, 2012
- Mutation in PNPT1 , which Encodes a Polyribonucleotide Nucleotidyltransferase, Impairs RNA Import into Mitochondria and Causes Respiratory-Chain DeficiencyThe American Journal of Human Genetics, 2012
- Correcting human mitochondrial mutations with targeted RNA importProceedings of the National Academy of Sciences, 2012
- Polynucleotide Phosphorylase Activity May Be Modulated by Metabolites in Escherichia coliJournal of Biological Chemistry, 2011
- Mitochondrial Enzyme Rhodanese Is Essential for 5 S Ribosomal RNA Import into Human MitochondriaJournal of Biological Chemistry, 2010
- Mechanisms of deadenylation‐dependent decayWIREs RNA, 2010
- PNPASE Regulates RNA Import into MitochondriaCell, 2010
- Human Mitochondrial SUV3 and Polynucleotide Phosphorylase Form a 330-kDa Heteropentamer to Cooperatively Degrade Double-stranded RNA with a 3′-to-5′ DirectionalityJournal of Biological Chemistry, 2009
- Crystal Structure of Escherichia coli Polynucleotide Phosphorylase Core Bound to RNase E, RNA and Manganese: Implications for Catalytic Mechanism and RNA Degradosome AssemblyJournal of Molecular Biology, 2009
- Polynucleotide Phosphorylase Protects Escherichia coli against Oxidative StressBiochemistry, 2009