Inheritance and expression of transgenes in kiwifruit

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Abstract
The inheritance and expression of transgenes was monitored in kiwifruit (Actinidia deliciosa (A.Chev.) C.F. Liang et A.R. Ferguson) established in a field trial. A gene encoding resistance to kanamycin and a reporter gene expressing the P‐glucuronidase (GUS) enzyme were both inherited and expressed in progeny of the kiwifruit plants. For two plants examined in detail, molecular evidence suggested that there were multiple copies of both the kanamycin resistance ( npt II) gene and the uid A gene. None of the actively expressing copies of npt II or uid A were linked. Expression of GUS was variable in some progeny plants, consistent with the idea that gene silencing phenomena were operating.

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