FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS
Authors:
Open Access
- 1 October 1972
- journal article
- Published by Rockefeller University Press in Journal of Cell Biology
- Vol. 55 (1) , 104-125
Abstract
0.3–0.5 M MgCl2 was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sectioning, freeze etching, and negative staining procedures.Keywords
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