Behavior of human osteoblast‐like cells in contact with electrodeposited calcium phosphate coatings

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Abstract
Calcium‐deficient hydroxyapatite (Ca‐def‐HAP) thin films were elaborated on Ti6Al4V substrates by electrodeposition. The coatings exhibit two different morphologies and crystallinities. Human osteoblast‐like cells (MG‐63) were cultured on the surfaces of these materials; the cell content and viability were evaluated up to 28 days. The scanning electron microscopy and biological investigations showed cells with a normal morphology, good proliferation, and viability from 7 to 21 days. But after 28 days, the number of live cells decreases in both cases; however, this decrease is less important in the case of calcium phosphate (CaP) coating surface when compared with the control (cell culture plastic). The cells cultured on Ca‐def‐HAP coating exhibit more cellular extensions and extracellular matrix. RT‐PCR for type I collagen, alkaline phosphatase, and osteocalcin studies were also carried out, and was found that the CaP enhances gene expression of ALP and OC and thus the differentiation of osteoblast‐like cells. Moreover, this study shows that the difference in the morphology of CaP coatings has no effect on the biocompatibility. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2006

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