Proposal of Sinomonas flava gen. nov., sp. nov., and description of Sinomonas atrocyanea comb. nov. to accommodate Arthrobacter atrocyaneus

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Abstract
A novel actinomycete strain, designated CW 108T, was isolated from a forest soil in Anhui Province, China. The cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. Chemotaxonomically, the peptidoglycan type was A3α, the cell-wall sugars contained galactose, mannose and ribose, the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylmonomethylethanolamine, the major fatty acids were ai-C15 : 0, i-C15 : 0 and ai-C17 : 0 and the predominant respiratory quinone was MK-9(H2), with a moderate amount of MK-8(H2) and a minor amount of MK-6(H2). The G+C content of the genomic DNA was 71.1 mol%. Phylogenetic analysis showed that strain CW 108T formed a deeply separated lineage in the family Micrococcaceae with Arthrobacter atrocyaneus DSM 20127T. The 16S rRNA gene sequence similarity between strain CW 108T and Arthrobacter atrocyaneus DSM 20127T was 99.4 %; sequence similarities between these strains and representatives of other species in the family Micrococcaceae with validly published names were lower than 95.2 %. DNA–DNA hybridization showed a level of relatedness of 52.2 % between the two neighbours. Based on the results of our polyphasic taxonomic study, strain CW 108T and Arthrobacter atrocyaneus DSM 20127T should be assigned to two different species of a novel genus within the family Micrococcaceae, for which the names Sinomonas flava gen. nov., sp. nov. and Sinomonas atrocyanea comb. nov. are proposed. The type strain of Sinomonas flava is CW 108T (=LMG 24447T =KCTC 19388T =CCTCC AB 207194T) and the type strain of Sinomonas atrocyanea is DSM 20127T =JCM 1329T =CGMCC 1.1891T =ATCC 13752T.

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