Microbial Metagenomics Evidence Reveals Forest Soil Amendment Contributes to Increased Sugarcane Yields in Long-Term Cropping Systems

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Abstract
Long-term continuous cropping is a prevalent agricultural practice aimed at maximizing land use efficiency and crop yields, yet it often leads to severe soil degradation, nutrient imbalance, and microbial community disruption. Effective soil remediation strategies are urgently needed to restore soil health and ensure sustainable agricultural production. In this study, we investigated the impact of forest soil amendment on microbial community structure, diversity, and functional potential in long-term continuous cropping soils. Using metagenomic sequencing, we analyzed soils from natural forest (BK), forest soil-amended soils (BCP), and fields under continuous cropping for 15 years (CP15) and 30 years (CP30). Forest soil amendment significantly mitigated microbial diversity loss and structural degradation caused by prolonged monoculture. Alpha diversity analysis revealed that BCP restored microbial diversity to levels comparable to BK, while beta diversity and NMDS analyses showed that microbial community composition in BCP closely resembled that of forest soil. Taxonomic profiling indicated that forest soil amendment enriched beneficial taxa such as Actinobacterota and Acidobacteriota, reversing shifts observed in CP15 and CP30. Functionally, COG and KEGG annotations revealed that BCP soils exhibited higher abundances of genes involved in carbohydrate metabolism, energy production, and nutrient cycling. Notably, the amendment reduced antibiotic resistance genes and virulence factors, potentially improving the microbial risk profile of soil communities. These findings demonstrate that forest soil amendment effectively restores microbial community structure and functionality in degraded soils, providing a nature-based solution for sustainable agriculture.
Funding Information
  • High-Level Science and Technology Talent and Innovation Team Selection Program (202205AD160014)
  • Science and Technology Program of Yunnan Province (202304BT090027)
  • Yunnan Fundamental Research Projects (202501AT070092)

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