MiR-15b Targets Cyclin D1 to Regulate Proliferation and Apoptosis in Glioma Cells

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Abstract
Aim. To investigate the role and mechanism of miR-15b in the proliferation and apoptosis of glioma.Methods. The miR-15b mimics were transfected into human glioma cells to upregulate the miR-15b expression. Cyclin D1 was determined by both western blotting analysis and luciferase reporter assay. Methylthiazol tetrazolium (MTT) and flow cytometry were employed to detect the cell proliferation, cell cycle, and apoptosis.Results. Overexpression of miR-15b inhibits proliferation by arrested cell cycle progression and induces apoptosis, possibly by directly targeting Cyclin D1. Both luciferase assay and bioinformatics search revealed a putative target site of miR-15b binding to the 3′-UTR of Cyclin D1. Moreover, expression of miR-15b in glioma tissues was found to be inversely correlated with Cyclin D1 expression. Enforced Cyclin D1 could abrogate the miR-15b-mediated cell cycle arrest and apoptosis.Conclusions. Our findings identified that miR-15b may function as a glioma suppressor by targeting the Cyclin D1, which may provide a novel therapeutic strategy for treatment of glioma.
Funding Information
  • National Natural Science Foundation of China (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • National Natural Science Foundation of China (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • Jiangsu Province’s Natural Science Foundation (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • Jiangsu Province’s Health Department (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • Yancheng Medical Science Development Foundation (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • Yancheng Medical Science Development Foundation (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • Jiangsu Province’s 333 Talent Program (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • Kunshan Social Development Foundation (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)
  • Kunshan Social Development Foundation (81201976, 81000963, BK2012670, z201318, YK2013003, YK2013019, BRA2011046, KS1006, KS1009)

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