Linking the chemical speciation of cerium to its bioavailability in water for a freshwater alga

Authors:
Publisher website
Google Scholar
Abstract
Over the past decade, researchers have begun to use metals of the lanthanide family for numerous applications, including liquid crystal display (LCD) screens, optical fibers, and laser technology. Unfortunately, little is presently known about their bioavailability or the mechanisms by which they might cause toxicity. The present study focuses on cerium (Ce), one of the most widely used lanthanides, and on validating the biotic ligand model as a means to predict Ce bioaccumulation. Short‐term exposures to Ce were performed using the unicellular alga Chlamydomonas reinhardtii, to better relate Ce bioavailability to its chemical speciation in solution. Maximum uptake fluxes (Jmax) and affinity constants for the binding of Ce to the biological uptake sites (KS) were established at pH 5.0 and pH 7.0. An apparent affinity constant of 1.8 × 107 M–1 was observed at pH 5.0, with a larger value obtained at pH 7.0 (6 × 107 M–1), albeit under conditions where equilibrium could not be confirmed. By evaluating Ce speciation using centrifugal ultrafiltration and single‐particle inductively coupled plasma spectrometry, it could be concluded that very little (∼30%) Ce was truly dissolved at pH 7.0, with the majority of the metal being present in colloidal species. Speciation was also monitored by fluorescence to evaluate Ce complexation by natural organic matter (NOM). The presence of NOM decreased Ce bioaccumulation in line with free Ce concentrations. Finally, competition with calcium for the metal uptake sites was shown to result in a decrease in Ce uptake by C. reinhardtii. Environ Toxicol Chem 2015;34:1711–1719. © 2015 SETAC
Funding Information
  • Environnement Canada
  • Fonds de Recherche du Québec - Nature et Technologies
  • Natural Sciences and Engineering Research Council of Canada

This publication has 44 references indexed in Scilit: