Supplementary Figure S3 from Differential Effects of High-Fiber and Low-Fiber Diets on Antitumor Immunity and Colon Tumor Progression in a Murine Model

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Abstract
Supplementary Figure S3 shows the impact of dietary fiber composition on immune cell subsets in ETBF-inoculated APCmin/+ mice. Flow cytometric (FACS) and CyTOF analyses were conducted to evaluate immune cell populations in the spleen, mesenteric lymph nodes (MLN), and lamina propria lymphocytes (LPLs) 8 weeks post-inoculation. Panels A–C display frequencies of neutrophils, monocytes, myeloid-derived suppressor cells, macrophages, and dendritic cells in the spleen, MLN, and LPLs, respectively. Panel D provides a tSNE plot from CyTOF analysis, highlighting major immune cell clusters in LPLs. Panels E–K and L–R quantify lymphocyte subsets, including Th1, Treg, Th17, CTL, and γδT17 cells in the spleen and MLN, respectively.