Optimization of the Polymer Coating for Glycosylated Gold Nanoparticle Biosensors to Ensure Stability and Rapid Optical Readouts

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Abstract
The development of new analytical tools to probe pathogenic infection processes and as point-of-care biosensors is crucial to combat the spread of infectious diseases or to detect biological warfare agents. Glycosylated gold nanoparticles that change color due to lectin (carbohydrate-binding protein) mediated aggregation may find use as biosensors but require a polymer coating between the particle surface and sugar to ensure stability in complex media. Here, RAFT polymerization is employed to generate glycosylated polymers to coat gold nanoparticles. Rather than being a passive component, it is shown here that the polymer coating has to be precisely tuned to achieve a balance between saline (steric) stability and speed of the readout. If the polymer is too long it can prevent or slow aggregation and hence lead to a poor readout in sensing assays. The optimized glyco-nanoparticles are also demonstrated to be useful for rapid detection of a ricin surrogate.
Funding Information
  • Engineering and Physical Sciences Research Council
  • Higher Education Funding Council for England

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