Figure 4 from Alprazolam Reduces Inflammatory Cytokine Production in Pancreatic Cancer–Associated Fibroblasts

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Abstract
ALP suppresses TLR4 signaling in PDAC CAFs. A, Heatmap of relative RNA expression levels for TLRs in a scRNA-seq profile of human fibroblasts from normal pancreas and PDAC tumor tissues analyzed in ref. (26). B, RT-qPCR validation of TLR4 RNA expression in C7-TA-PSC cells at baseline relative to GAPDH. C, Fold change in cytokine RNA expression levels determined by RT-qPCR following 24 hours of treatment with DMSO, 20 μmol/L ALP, and/or 2.5 μg/mL LPS. Experiments in B and C were conducted in technical and biological triplicate (n = 3). Data are represented as mean ± SD. Statistical analyses in C were conducted using one-way ANOVA with a Tukey correction for multiple comparisons. ns, not significant; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.