Fast Track to Discover Novel Promoters in Rice

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Abstract
Promoters are key components for the application of biotechnological techniques in crop plants. Reporter genes such as GUS or GFP have been used to test the activity of promoters for diverse applications. A huge number of T-DNAs carrying promoterless GUS near their right borders have been inserted into the rice genome, and 105,739 flanking sequence tags from rice lines with this T-DNA insertion have been identified, establishing potential promoter trap lines for 20,899 out of 55,986 genes in the rice genome. Anatomical meta-expression data and information on abiotic stress related to these promoter trap lines enable us to quickly identify new promoters associated with various expression patterns. In the present report, we introduce a strategy to identify new promoters in a very short period of time using a combination of meta-expression analysis and promoter trap lines.
Funding Information
  • Korea Institute of Marine Science and Technology promotion (No. 20180430 to JKH)
  • National Research Foundation of Korea (2018R1A6A3A11050326 to YHY)
  • Next-Generation BioGreen 21 Program (PJ01325901, and PJ01366401 to KHJ)

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