Single-cell-based system to monitor carrier driven cellular auxin homeostasis
Open Access
- 4 February 2013
- journal article
- Published by Springer Nature in BMC Plant Biology
- Vol. 13 (1) , 20
Abstract
Abundance and distribution of the plant hormone auxin play important roles in plant development. Besides other metabolic processes, various auxin carriers control the cellular level of active auxin and, hence, are major regulators of cellular auxin homeostasis. Despite the developmental importance of auxin transporters, a simple medium-to-high throughput approach to assess carrier activities is still missing. Here we show that carrier driven depletion of cellular auxin correlates with reduced nuclear auxin signaling in tobacco Bright Yellow-2 (BY-2) cell cultures.Keywords
This publication has 69 references indexed in Scilit:
- Emergence of tissue polarization from synergy of intracellular and extracellular auxin signalingMolecular Systems Biology, 2010
- ABP1 Mediates Auxin Inhibition of Clathrin-Dependent Endocytosis in ArabidopsisCell, 2010
- The march of the PINs: developmental plasticity by dynamic polar targeting in plant cellsThe EMBO Journal, 2010
- Auxin influx inhibitors 1-NOA, 2-NOA, and CHPAA interfere with membrane dynamics in tobacco cellsJournal of Experimental Botany, 2010
- Cellular Responses to Auxin: Division versus ExpansionCold Spring Harbor Perspectives in Biology, 2010
- A regulated auxin minimum is required for seed dispersal in ArabidopsisNature, 2009
- Auxin transport through non-hair cells sustains root-hair developmentNature Cell Biology, 2008
- Differential degradation of PIN2 auxin efflux carrier by retromer-dependent vacuolar targetingProceedings of the National Academy of Sciences, 2008
- Auxin acts as a local morphogenetic trigger to specify lateral root founder cellsProceedings of the National Academy of Sciences, 2008
- Cytokinin and auxin interaction in root stem-cell specification during early embryogenesisNature, 2008