Lon domain‐containing protein 1 represses thioredoxin y2 and regulates ROS levels in Arabidopsis chloroplasts
Open Access
- 8 November 2019
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 594 (6) , 986-994
Abstract
Plant thioredoxins (Trxs) act as antioxidants and function as redox regulators in the chloroplast. Although the regulation of ROS in chloroplasts is well elucidated, the precise regulation mechanism of Trx remains unknown. Here, we characterize a novel chloroplast protein, Lon domain‐containing protein 1 (LCP1), which contains only a Lon domain, the precise function of which is not known. We find that LCP1 interacts with Trx‐y2 and represses its activity, and that knockdown (KD) of LCP1 causes anther indehiscence due to deficient lignin deposition. In addition, LCP1 KD plants show less ROS accumulation and lower expression of ROS‐responsive marker genes than the wild‐type plant. Taken together, we suggest that LCP1 directly regulates Trx‐y2 and controls H2O2 levels and, thereby, regulates lignin polymerization in the anther endothecium.Keywords
Funding Information
- National Science Foundation, United Arab Emirates (2014R1A2A2A01005974, 2019R1F1A1060014)
- Rural Development Administration (PJ01367001)
- Korea University
This publication has 30 references indexed in Scilit:
- Distinct quaternary structures of the AAA+ Lon protease control substrate degradationProceedings of the National Academy of Sciences, 2013
- A Cystathionine-β-Synthase Domain-Containing Protein, CBSX2, Regulates Endothecial Secondary Cell Wall Thickening in Anther DevelopmentPlant and Cell Physiology, 2012
- Multitasking in the mitochondrion by the ATP-dependent Lon proteaseBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2012
- Single Cystathionine β-Synthase Domain–Containing Proteins Modulate Development by Regulating the Thioredoxin System in ArabidopsisThe Plant Cell, 2011
- Arabidopsis MYB26/MALE STERILE35Regulates Secondary Thickening in the Endothecium and Is Essential for Anther DehiscenceThe Plant Cell, 2007
- NAC Transcription Factors, NST1 and NST3, Are Key Regulators of the Formation of Secondary Walls in Woody Tissues ofArabidopsisThe Plant Cell, 2007
- SND1, a NAC Domain Transcription Factor, Is a Key Regulator of Secondary Wall Synthesis in Fibers ofArabidopsisThe Plant Cell, 2006
- Characterization of thioredoxin y, a new type of thioredoxin identified in the genome of Chlamydomonas reinhardtiiFEBS Letters, 2003
- Anther developmental defects in Arabidopsis thaliana male-sterile mutantsSexual Plant Reproduction, 1999
- Anther dehiscence in Lycopersicon esculentum Mill. I. Structural aspectsNew Phytologist, 1989