Cysteine conjugate β-lyase in the gastrointestinal bacterium Fusobacterium necrophorum
Authors:
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 13 (11) , 689-700
Abstract
1. A cysteine conjugate β-lyase from the anaerobic gastrointestinal bacterium Fusobacterium necrophorum was purified 51-fold by heat treatment, ammonium sulphate fractionation, gel-filtration chromatography, and anion-exchange chromatography. 2. This enzyme catalyses the cleavage of the thioether linkage in cysteine conjugates of the following S-alkyl- or S-aryl-linked compounds: cysteine conjugate of propachlor (2-S-cysteinyl-N-isopropylacetanilide); 1,2-dihydro-1-hydroxy-2-S-cysteinylnaphthalene and S-(2-benzothiazolyl)cysteine. 3. 2-Mercapto-N-isopropylacetanilide, pyruvic acid and ammonia were produced from the β-lyase cleavage of the cysteine conjugate of propachlor in equimolar ratios. 4. The apparent Km values for the cysteine conjugate of propachlor and S-(benzothiazolyl)cysteine were 1.1 and 1.0mM, respectively. 5. Pyridoxal phosphate was required for enzymic activity. Ammonium ion activated enzymic activity, while hydroxylamine completely inhibited the enzyme. Dithiothreitol and bovine serum albumin had no effect on enzymic activity.Keywords
This publication has 15 references indexed in Scilit:
- Fragmentation patterns of trimethylsilyl derivatives of dihydrodiol glucuronides produced by metabolism of naphthalene and 1-methyl-N-naphthylcarbamateJournal of Mass Spectrometry, 1982
- Role of Gut Microflora in Metabolism of Glutathione Conjugates of Xenobiotics1981
- S .fwdarw. N and N .fwdarw. S reverse rearrangement of S- and N-(2,4-dinitrophenyl)cysteinesThe Journal of Organic Chemistry, 1981
- A new metabolite of propachlor isolated from germfree rat excretaJournal of Mass Spectrometry, 1981
- Metabolism of Propachlor by the Germfree RatScience, 1980
- In vitro metabolism of pentachloronitrobenzene to pentachloromethylthiobenzene by onion: Characterization of glutathione S-transferase, cysteine C-S lyase, and S-adenosylmethionine methyl transferase activitiesPesticide Biochemistry and Physiology, 1980
- Metabolism of 2‐chloro‐N‐isopropylacetanilide (Propachlor) in the ratJournal of Environmental Science and Health, Part B, 1979
- Replacement of a chlorine with a methylsulfonyl group in the metabolism of propachlor (2-chloro-N-isopropylacetanilide)Journal of Mass Spectrometry, 1976
- Mercapturic acid formation in the metabolism of propachlor, CDAA, and fluorodifen in the ratPesticide Biochemistry and Physiology, 1975
- The biotransformation of a sulfonamide to a mercaptan and to mercapturic acid and glucuronide conjugatesBiochemical Pharmacology, 1965