Extracellular alpha satellite DNA in human plasma as a novel molecular biomarker for early diagnosis of bladder cancer

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Abstract
Although bladder cancer (BC) is a common urological disease, there are deficiencies in current methods used for its detection, hence the research on the identification of non- invasive biomarkers is given a high priority. Alpha satellite DNA is a major human satellite DNA (hASAT), which is important for genome stability and whose aberrant overexpression is a hallmark of many human cancers. Here we identified and quantified its release into the circulation of BC patients, demonstrating its increased copy number with respect to healthy controls. Absolute quantification of extracellular alpha satellite DNA (ecASAT) repeats was performed by nanoplate-based digital PCR using few microliters of blood plasma from cancer patients as well as from BC negative controls. We found that copy number of ecASAT repeats is significantly higher in blood plasma of BC patients than in healthy controls. The results suggest that the ec-hASAT copy number variation in human plasma could act as a reliable biomarker for early detection of BC.

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