Biomolecule Sensor Based on Azlactone‐Modified Hydrogel Films

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Abstract
A 3D hydrogel layer is probed by combining surface plasmon resonance with optical waveguide spectroscopy to detect biomolecules. A template terpolymer P(DMAAm‐co‐DMIAAm‐co‐VDMA) is synthesized via reversible addition‐fragmentation chain‐transfer polymerization. The terpolymer is then modified with an amino group bearing biotin to enable biomolecular recognition for streptavidin. A hydrogel thin layer is prepared onto a gold surface after spin‐coating and photo‐crosslinking of the modified polymer. Finally, the hydrogel is utilized to quantitatively detect streptavidin by using surface plasmon resonance–optical waveguide spectroscopy measurements.

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