Use of Reverse Transcriptase PCR To Subtype N1 to N9 Neuraminidase Genes of Avian Influenza Viruses
Authors:
Open Access
- 1 July 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (7) , 2301-2303
Abstract
Reverse transcriptase PCR designed to amplify the N1 to N9 neuraminidase (NA) genes of avian influenza viruses detected 118 of the 119 NA genes tested (99.2%) in a subtype-specific manner. This technique successfully subtyped all 167 recent avian influenza viruses isolated from birds. Subtype specificity was confirmed by sequence analyses of all 285 PCR products.Keywords
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