Indeterminate domain 10regulates ammonium‐mediated gene expression in rice roots

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Abstract
Summary: Indeterminate domain(IDD) genes are a family of plant transcriptional regulators that function in the control of development and metabolism during growth. Here, the function ofOryza sativa indeterminate domain 10(OsIDD10) has been explored in rice plants. Compared with wild‐type roots,idd10mutant roots are hypersensitive to exogenous ammonium. This work aims to define the action ofIDD10on gene expression involved in ammonium uptake and nitrogen (N) metabolism. The ammonium induction of key ammonium uptake and assimilation genes was examined in the roots ofidd10mutants andIDD10overexpressors. Molecular studies and transcriptome analysis were performed to identify target genes and IDD10 bindingcis‐elements. IDD10 activates the transcription ofAMT1;2andGDH2by binding to acis‐element motif present in the promoter region ofAMT1;2and in the fifth intron ofGDH2. IDD10 contributes significantly to the induction of several genes involved inN‐linked metabolic and cellular responses, including genes encoding glutamine synthetase 2, nitrite reductases and trehalose‐6‐phosphate synthase. Furthermore, the possibility that IDD10 might influence the N‐mediated feedback regulation of target genes was examined. This study demonstrates that IDD10 is involved in regulatory circuits that determine N‐mediated gene expression in plant roots.
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  • Rural Development Administration

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