Mass Spectrometry-Based Analysis of IGF-1 and hGH
Authors:
- Mario ThevisMTMario Thevis
- Michael BredehöftMBMichael Bredehöft
- Maxie KohlerMKMaxie Kohler
- Wilhelm SchänzerWSWilhelm Schänzer
- 17 September 2009
- book chapter
- Published by Springer Nature
- No. 195,p. 201-207
Abstract
Mass spectrometric approaches have been used to determine various peptide hormones in sports drug testing. While insulin-like growth factor-1 (IGF-1) and its synthetic analogs are qualitatively and/or quantitatively measured by liquid chromatography–tandem mass spectrometry after immunoaffinity purification, methods of uncovering doping rule violations with illegal applications of human growth hormone (hGH) have not yet been established using mass spectrometry-based assays. However, substantial information on the heterogeneity of hGH, splice variants and post-translational modifications with respective locations as elucidated by mass spectrometry are of utmost importance for improving currently employed immunological procedures.Keywords
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