A Negative Regulatory Element and Its Binding Protein in the Upstream of Enhancer II of Hepatitis B Virus
Authors:
- Yong Won YiYYYong Won Yi
- Cheol Yong ChoiCCCheol Yong Choi
- Hyune Mo RhoHRHyune Mo Rho
- 1 December 1997
- journal article
- research article
- Published by SAGE Publications in DNA and Cell Biology
- Vol. 16 (12) , 1459-1465
Abstract
The hepatitis B virus (HBV) core/pregenomic promoter is regulated by enhancer I (ENI) and enhancer II (ENII) which are located upstream of the initiation sites of core/pregenomic transcripts. In this study, we identified a negative regulatory element (NRE) (nt 1576 to 1639) upstream of ENII by serial deletion analysis; a 33 kDa cellular protein in HepG2 cells binds to this element. The NRE has a significant activity if it is located upstream of ENII in HepG2 cells. Mutational analysis showed that the sequence (5′-CCAC-3′) from nt 1612 to 1615 is responsible for the repression activity of NRE. Southwestern blotting and UV-crosslinking assays with HepG2 nuclear extracts also demonstrated that the 33 kDa protein in HepG2 cells binds to the sequence. It, thus, appears that the 33 kDa protein is responsible for the repression activity of NRE.Keywords
This publication has 30 references indexed in Scilit:
- The retinoblastoma gene product activates transcription of the hepatitis B virus pregenomic promoter through the Sp1 binding sitesJournal of General Virology, 1996
- A Positive Regulatory Sequence of Hepatitis B Viral Small X PromoterEuropean Journal of Biochemistry, 1996
- Release of the Hepatitis β Virus-associated DNA Polymerase from the Viral Particle by the Proteolytic CleavageJournal of Biological Chemistry, 1995
- Characterization of a Functional Hepatocyte Nuclear Factor 3 Binding Site in the Hepatitis B Virus Nucleocapsid PromoterVirology, 1995
- Regulation of Hepatitis B Virus ENI Enhancer Activity by Hepatocyte-Enriched Transcription Factor HNF3Virology, 1994
- Effect of frameshift mutation in the pre-C region of hepatitis B virus on the X and C genesJournal of General Virology, 1994
- Mutational Analysis of Hepatitis B Virus Enhancer 2Virology, 1993
- Replication of a mutant hepatitis B virus with a fused X-C reading frame in hepatoma cellsJournal of General Virology, 1992
- Repression of the hepatitis B virus enhancer by a cellular factorJournal of General Virology, 1992
- Replication of the genome of a hepatitis B-like virus by reverse transcription of an RNA intermediateCell, 1982