Observation of single‐stranded DNA on mica and highly oriented pyrolytic graphite by atomic force microscopy
Authors:
- Dmitry V. KlinovDKDmitry V. Klinov
- Guillaume WitzGWGuillaume Witz
- Sergey K. SekatskiiSSSergey K. Sekatskii
- Giovanni DietlerGDGiovanni Dietler
- 18 September 2006
- journal article
- Published by Wiley in FEBS Letters
- Vol. 580 (24) , 5671-5675
Abstract
Atomic force microscopy was used to image single‐stranded DNA (ssDNA) adsorbed on mica modified by Mg2+, by 3‐aminopropyltriethoxysilane or on modified highly oriented pyrolytic graphite (HOPG). ssDNA molecules on mica have compact structures with lumps, loops and super twisting, while on modified HOPG graphite ssDNA molecules adopt a conformation without secondary structures. We have shown that the immobilization of ssDNA under standard conditions on modified HOPG eliminates intramolecular base‐pairing, thus this method could be important for studying certain processes involving ssDNA in more details.Keywords
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