Abnormal Enamel Mineralization and Hypercalcemia due to GNA11 Variants

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Abstract
Abnormal enamel mineralization has been reported in genetic conditions associated with hypocalcemia but never with hypercalcemia. In the present study, we report 10 patients from 2 unrelated families in whom hypomineralized enamel co-segregated with hypercalcemia due to a complete deletion or frameshifting premature truncation (p.(Ser18Argfs*2)) of the guanine nucleotide–binding protein (G-protein) subunit α 11 ( GNA11 ) gene. Both GNA11 variants were heterozygous and predicted to cause a loss of function of Gα 11 . Multiproxy imaging analyses of naturally exfoliated primary teeth and clinically extracted permanent teeth from 2 affected patients revealed abnormal enamel mineralization, with constitutive patches of hypomineralization following no set pattern. In parallel, the study of wild-type mouse tooth germs using combined molecular and protein analyses showed Gna11 and Gα 11 protein expression in ameloblasts at the secretion and maturation stages, as well as in odontoblasts, suggesting a role for Gα 11 in tooth formation. Furthermore, the analysis of a mouse model of Familial Hypocalciuric Hypercalcemia 2 (FHH2) revealed delayed onset of enamel mineralization in the continuously growing incisors and hypomineralized enamel in both incisor and molars of heterozygous ( Gna11 Tm1b +/− ) mice, with defects that are very similar to those affecting human FHH2 teeth. In conclusion, our study reveals that abnormal enamel mineralization may occur in association with hypercalcemia due to loss-of-function GNA11 mutations, opening a new field of investigation and highlighting the need to include a dentist in the multidisciplinary team in charge of patients with monogenic calcium disorders.
Funding Information
  • Fonds De La Recherche Scientifique - FNRS (1226325N)
  • Infrastructures en Biologie Santé et Agronomie
  • Agence Nationale de la Recherche (ANR-25-CE17-1624)
  • Agence Nationale de la Recherche (FHU DDS net)
  • Fonds Wetenschappelijk Onderzoek (VS00125N)
  • France Life Imaging (ANR-11-INBS-0006)

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