Analyzing Plant Signaling Phospholipids Through 32Pi-Labeling and TLC
- 21 March 2013
- book chapter
- Published by Springer Nature
- Vol. 1009, 3-15
Abstract
Lipidomic analyses through LC-, GC-, and ESI-MS/MS can detect numerous lipid species based on headgroup and fatty acid compositions but usually miss the minor phospholipids involved in cell signaling because of their low chemical abundancy. Due to their high turnover, these signaling lipids are, however, readily picked up by labeling plant material with 32P-orthophosphate and subsequent analysis of the lipid extracts by thin layer chromatography. Here, protocols are described for suspension-cultured tobacco BY-2 cells, young Arabidopsis seedlings, Vicia faba roots, and Arabidopsis leaf disks, which can easily be modified for other plant species and tissues.Keywords
This publication has 28 references indexed in Scilit:
- Involvement of phospholipase D and phosphatidic acid in the light-dependent up-regulation of sorghum leaf phosphoenolpyruvate carboxylase-kinaseJournal of Experimental Botany, 2010
- Plant PA signaling via diacylglycerol kinaseBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2009
- Phospholipid Signaling Responses in Salt-Stressed Rice LeavesPlant and Cell Physiology, 2009
- Substrate preference of stress‐activated phospholipase D in Chlamydomonas and its contribution to PA formationThe Plant Journal, 2003
- Phospholipase D in Phytophthora infestans and Its Role in Zoospore EncystmentMolecular Plant-Microbe Interactions®, 2002
- Profiling Membrane Lipids in Plant Stress ResponsesJournal of Biological Chemistry, 2002
- In vivo substrates and the contribution of the common phospholipase D, PLDα, to wound-induced metabolism of lipids in ArabidopsisBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2001
- Water Deficit Triggers Phospholipase D Activity in the Resurrection Plant Craterostigma plantagineumThe Plant Cell, 2000
- Activation of phospholipase D by calmodulin antagonists and mastoparan in carnation petalsJournal of Experimental Botany, 1997
- Identification of Diacylglycerol Pyrophosphate as a Novel Metabolic Product of Phosphatidic Acid during G-protein Activation in PlantsJournal of Biological Chemistry, 1996