Lipopolysaccharide elicits expression of immune‐related genes in the silkworm, Bombyx mori
Authors:
- 19 January 2009
- journal article
- Published by Wiley in Insect Molecular Biology
- Vol. 18 (1) , 71-75
Abstract
Lipopolysaccharide (LPS), a major cell wall component of Gram‐negative bacteria, was found to be unable to activate immune‐related genes in Drosophila melanogaster . In contrast, highly purified LPS elicited immune‐related gene expression in the fat body of Bombyx mori . However, the level of activation by highly purified LPS was lower than crude LPS and peptidoglycan. Furthermore, synthetic lipid A also activated these genes, suggesting that B. mori possesses unknown signal pathways to activate immune‐related genes by LPS. Up‐regulation of antimicrobial peptide genes by highly purified LPS was not confirmed in the immune‐responsive cell line, NIAS‐Bm‐aff3, suggesting that some factors necessary for signal transduction activated by LPS are deficient in this cell line.Keywords
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