An mRNA degrading complex in Rhodobacter capsulatus
Author:
- 15 November 2001
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 29 (22) , 4581-4588
Abstract
An RNA degrading, high molecular weight complex was purified from Rhodobacter capsulatus. N-terminal sequencing, glycerol-gradient centrifugation, and immunoaffinity purification as well as functional assays were used to determine the physical and biochemical characteristics of the complex. The complex comprises RNase E and two DEAD-box RNA helicases of 74 and 65 kDa, respectively. Most surprisingly, the transcription termination factor Rho is a major, firmly associated component of the degradosome.Keywords
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