The Effect of Subacute Exposure to Acrylamide on Femoral Bone Microstructure in Laboratory Mice

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Abstract
Acrylamide, a substituted unsaturated hydrocarbon, is often found in fried potatoes, flour, baby food, coffee, and olives. It is one of the most commonly consumed neurotoxins by humans. To date, its effect on bone structure has not been investigated in experimental animals. The aim of our manuscript was to analyze the impact of subacute exposure to acrylamide on the microscopic compact and trabecular bone tissue structures in laboratory mice. Adult male mice were divided into two groups. Animals from the experimental group (group E, n = 4) were treated perorally with two doses of acrylamide (1 mg/kg b.w.) during 24 hours. The group without administration of AA served as a control (group C, n = 4). Three hours after the second dose of AA (after 27 hours), mice were killed and their femurs were used for microscopic analysis. Our results demonstrate that subacute exposure to acrylamide causes an absence of the primary vascular radial bone tissue in pars posterior of the endosteal border. Mice from E group had more intact secondary osteons in pars medialis of the middle part of compact bone. Also, a few resorption lacunae were found in pars anterior of periosteal borders in these mice. Subacute exposure to AA significantly decreased the size of the primary osteon vascular canals (P<0.05) in compact bone tissue. In trabecular bone tissue, the values for bone volume, trabecular number, and bone surface were significantly increased in mice from the E group. In contrast, the value for trabecular separation was significantly decreased in these mice.