A new technique for the study of temporal bone pathology
Authors:
- L. MichaelsLML. Michaels
- Meher WellsMWMeher Wells
- A. FrohlichAFA. Frohlich
- 1 April 1983
- journal article
- research article
- Published by Wiley in Clinical Otolaryngology
- Vol. 8 (2) , 77-85
Abstract
A new technique for the study of the temporal bone at autopsy [in humans] is presented in which thin slices of the fixed bone are prepared on a special cutting machine which preserves the configuration of the delicate anatomical structures. Selected areas are then processed for light microscopy. There is thus opportunity for gross examination, only a short period for immersion in acid for decalcification if required, and special histological procedures may also be carried out. The method is quick and technically easier than serial sectioning and storage of the smaller numbers of sections from each case is less of a problem. Temporal bones which were perfused through the perilymph within 3 h of death may also be cut in the same way in order to allow removal of samples of membranous labyrinth for surface preparation or EM, avoiding the need for drilling to reach the membranous labyrinth, and being quicker and less damaging.Keywords
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