MiR-221 is involved in depression by regulating Wnt2/CREB/BDNF axis in hippocampal neurons

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Abstract
Objective: The aim of this study was to investigate the mechanism of miR-221 in depression. Methods: The molecules expressions were measured by qRT-PCR and western blot. The sucrose preference test (SPT), forced swimming test (FST) and tail suspension test (TST) were used to detect depressive-like symptoms. MTT assay and flow cytometric was used to measure the proliferation and apoptosis of hippocampal neuronal. Results: MiR-221 expression in the cerebrospinal fluid and serum of major depressive disorder patients and the hippocampus of chronic unpredictable mild stress (CUMS) mice were increased, while the expression of Wnt2, p-CREB and BDNF were decreased. Additionally, silence of miR-221 increased sucrose preference of CUMS mice and shortened the immobility time of CUMS mice in SPT and FST. MiR-221 could targeted regulate Wnt2, and knockdown of Wnt2 reversed the effect of miR-221 inhibitor on the proliferation and apoptosis of hippocampal neurons and countered the promoting effect of miR-221 inhibitor on the expression of Wnt2, p-CREB and BDNF. Conclusion: MiR-221 could promote the development of depression by regulating Wnt2/CREB/BDNF axis.
Funding Information
  • National Natural Science Foundation of China (No.81571318 to XS)
  • School and Hospital Co-incubation Funds (No.2017-BSTDJJ-04 to XS)
  • Department of Science and Technology of Henan Province (No.2017JQ023)
  • Health and Family Planning Commission of Henan Province (No.201501015 to XS)
  • The National Natural Science Foundation of China (No.81401110 to XL)
  • Department of Science and Technology of Henan Province (No. 162102410061 to XS)

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