Chimeric RNA/DNA oligonucleotide-directed gene targeting in rice
Authors:
- 1 February 2004
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 22 (7) , 509-512
Abstract
Site-specific mutagenesis in a rice genome was obtained by introducing chimeric RNA/DNA oligonucleotides (COs) by means of particle bombardment. Three COs were designed to target the independent codons for Pro-171, Trp-548 and Ser-627 of the endogenous rice acetolactate synthase (ALS) gene so it would confer resistance to ALS-inhibiting herbicides. Sequencing of the ALS gene of herbicide-resistant plants demonstrated that the ALS sequence was modified in a site-specific fashion. The efficiency of gene conversion mediated by COs was estimated to be 1×10-4. These results demonstrate that CO-directed gene targeting is feasible in rice.Keywords
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