Near Infrared Emitting Semiconductor Polymer Dots for Bioimaging and Sensing

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Abstract
Semiconducting polymer dots (Pdots) are rapidly becoming one of the most studied nanoparticles in fluorescence bioimaging and sensing. Their small size, high brightness, and resistance to photobleaching make them one of the most attractive fluorophores for fluorescence imaging and sensing applications. This paper highlights our recent advances in fluorescence bioimaging and sensing with nanoscale luminescent Pdots, specifically the use of organic dyes as dopant molecules to modify the optical properties of Pdots to enable deep red and near infrared fluorescence bioimaging applications and to impart sensitivity of dye doped Pdots towards selected analytes. Building on our earlier work, we report the formation of secondary antibody-conjugated Pdots and provide Cryo-TEM evidence for their formation. We demonstrate the selective targeting of the antibody-conjugated Pdots to FLAG-tagged FLS2 membrane receptors in genetically engineered plant leaf cells. We also report the formation of a new class of luminescent Pdots with emission wavelengths of around 1000 nm. Finally, we demonstrate the formation and utility of oxygen sensing Pdots in aqueous media.
Funding Information
  • US Department of Energy Basic Energy Research (DOE-BER) (DE-SC0020346)
  • US Department of Energy (DOE) (FWP 74562, FWP 74915)
  • US national Institute of Health (NIH) Chemistry-Biology Interface (CBI) (NIH/NIGMS T32 GM066706)
  • US National Science Foundation (NSF) (CHE-1904600)
  • National Science Foundation (NSF) Plant Genome Program (IOS-2048410)
  • Environmental Molecular Sciences Laboratory, a DOE Office of Science User Facility sponsored by the Biological and Environmental Research program (DE-AC05-76RL01830)

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