Enrichment culture can bias the isolation ofCampylobactersubtypes

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Abstract
SUMMARY: Enrichment culture is often used to isolateCampylobacter. This study compared isolation ofCampylobacterspp. from 119 broiler chicken environments from two farms, using Preston and modified Exeter (mExeter) and modified Bolton (mBolton) enrichments. mExeter was significantly more effective in isolatingCampylobacterspp. from the environmental samples compared to Preston (PP0·05). Enrichment broth type did not affect isolation from chicken faecal or soil and litter samples.C. jejuniwas isolated from significantly more environmental samples using mExeter broth compared to Preston (PP0·05). OnlyC. coliwas isolated from the soil and litter samples and although bothC. jejuniandC. coliwere recovered from the faecal samples there was no effect of using different enrichment broths. The majority of samples where the same species had been isolated yielded the same or closely related genotypes as defined by pulsed-field gel electrophoresis. Isolates recovered using Preston and mBolton broths were less genetically diverse than those from mExeter broth. We conclude that the enrichment method used affects both the number and species ofCampylobacterisolated from naturally contaminated samples.

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