Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea

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Abstract
Two Gram-positive or Gram-variable, endospore-forming, moderately halophilic rods (strains TF-11T and TF-12T) were isolated from a tidal flat of the Yellow Sea in Korea and were subjected to a polyphasic taxonomic study. Strains TF-11T and TF-12T had cell-wall peptidoglycan based on meso-diaminopimelic acid. The predominant menaquinone found in the two strains was MK-7. The cellular fatty acid profiles of both strains contained large amounts of branched and saturated fatty acids. The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0. The DNA G+C contents of strains TF-11T and TF-12T were respectively 49 and 38 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strains TF-11T and TF-12T fell within the radiation of the cluster comprising Bacillus species. The level of 16S rDNA sequence similarity between strains TF-11T and TF-12T was 98·3 %. Strains TF-11T and TF-12T exhibited levels of 16S rDNA sequence similarity of less than 96·0 and 96·3 %, respectively, to Bacillus species. The mean level of DNA–DNA relatedness between the two strains was approximately 7 %. On the basis of phenotypic and phylogenetic data and genomic distinctiveness, strains TF-11T and TF-12T should be placed in the genus Bacillus as two distinct novel species, for which the names Bacillus marisflavi sp. nov. (type strain TF-11T=KCCM 41588T=JCM 11544T) and Bacillus aquimaris sp. nov. (type strain TF-12T=KCCM 41589T=JCM 11545T) are proposed.
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