Immunization with Lentivirus-Like Particles Elicits a Potent SIV-Specific Recall Cytotoxic T-Lymphocyte Response in Rhesus Monkeys

Authors:
  • GMGail P. Mazzara
    Gail P. Mazzara
  • SDSteven O. DiSCIULLO
    Steven O. DiSCIULLO
  • DPDennis L. Panicali
    Dennis L. Panicali
  • NLNorman L. Letvin
    Norman L. Letvin
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Abstract
An effective acquired immunodeficiency syndrome (AIDS) vaccine should be capable of eliciting human immunodeficiency virus (HlV)-specific cytotoxic T-lymphocyte (CTL) responses. We have explored the use of lentivirus-like particles produced in mammalian cells infected with a recombinant vaccinia virus to immunize for the induction of CTL in the simian immunodeficiency virus (SIV)/rhesus monkey model for AIDS. SIV-like particles in a threonyl-MDP-based adjuvant did not elicit a high frequency of SIV gag -specific effector cells in naive rhesus monkeys. However, immunization with these particles elicited a potent recall CTL response in monkeys previously vaccinated with a recombinant vaccinia-SIV virus. These observations suggest that poxvirus-produced virus-like particles may represent a safe immunogen for use in periodic boosting to maintain viable cell-mediated immunity to the AIDS virus.

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