Pest-Managing Efficacy of trans-Asarone Isolated from Daucus carota L. Seeds

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Abstract
The bioactive hexane extract of Daucus carota seed yielded 2,4,5-trimethoxybenzaldehyde (1), oleic acid (2), trans-asarone (3), and geraniol (4). Compounds 1−4 were evaluated for their mosquitocidal, nematicidal, antifeedant, and antimicrobial activities. Only trans-asarone was active in the assays performed, causing 100% mortality to fourth-instar mosquito larvae, Aedes aegyptii, at 200 μg mL-1 and the nematodes Caenorhabditis elegans and Panagrellus redivivus at 100 μg mL-1. In feeding trials, trans-asarone also caused significant weight reductions of the caterpillars Helicovarpa zea, Heliothis virescens, and Manduca sexta when incorporated into artificial diet at a concentration of 100 μg mL-1. Also, it exhibited slight activity at 100 μg mL-1 against the yeasts Candida albicans, Candida parapsilasis, and Candida kruseii. Keywords: Daucus carota; Umbelliferae; trans-asarone; insecticidal; mosquitocidal; nematicidal; antifeedant; antimicrobial

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