Concanavalin A induces microtubule assembly and specific granule discharge in human polymorphonuclear leukocytes.
Authors:
- S HoffsteinSHS Hoffstein
- R SobermanRSR Soberman
- I GoldsteinIGI Goldstein
- G WeissmannGWG Weissmann
Open Access
- 1 March 1976
- journal article
- Published by Rockefeller University Press in Journal of Cell Biology
- Vol. 68 (3) , 781-787
Abstract
Human neutrophils stimulated by concanavalin A (Con A, 100 microng/ml) contained markedly enhanced numbers of microtubules and discharged peroxidase-negative (specific) but not peroxidase-position (azurophile) granules. Release of lysozyme from specific granules was dose and time dependent, could be inhibitied by alpha-methyl-D-mannoside, and enhanced by cytochalasin B. Many microtubules were associated with internalized plasma membrane bearing Con A binding sites.Keywords
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