Streptomyces griseus aminopeptidase is a calcium‐activated zinc metalloprotein
Authors:
- 1 August 1989
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 183 (2) , 471-477
Abstract
A heat‐stable aminopeptidase with an N‐terminal Ala‐Pro‐Asp‐Ile‐Pro‐Leu sequence has been purified from Streptomyces griseus by heat treatment followed by gel‐exclusion and anion‐exchange chromatographic procedures. The enzyme is a monomeric zinc metalloenzyme showing an apparent molecular mass of 33 kDa by sodium dodecyl sulfate/polyacrylamide gel electrophoresis and 21 kDa by gel filtration on Superose 12.Calcium ions bind to the enzyme, pKCa 4.5, and activate it about sixfold when the substrate is leucine‐4‐nitroanilide (0.4 mM in 50 mM Tris/HCl pH 8.0, 25°C). Binding of Ca2+ also contributes to the thermal stability of the protein. This aminopeptidase may be useful for two‐stage assays of bacterial and mammalian metalloendopeptidases; it may also serve in studies of proteolytic enzyme activation by calcium ions.Keywords
This publication has 32 references indexed in Scilit:
- Enzymatic Cleavage of Amino Terminal Methionine from Recombinant Human Interleukin 2 and Growth Hormone by Aminopeptidase MNature Biotechnology, 1987
- Inactivation and Metabolism of NeuropeptidesAnnual Review of Neuroscience, 1986
- Spectral and kinetic studies of metal-substituted Aeromonas aminopeptidase: nonidentical, interacting metal-binding sitesBiochemistry, 1985
- Kinetic parameters of metal-substituted leucine aminopeptidase from bovine lensBiochemistry, 1983
- Membrane-bound kidney neutral metalloendopeptidase: interaction with synthetic substrates, natural peptides, and inhibitorsBiochemistry, 1983
- Properties of “enkephalinase” from rat kidney: Comparison of dipeptidyl-carboxypeptidase and endopeptidase activitiesBiochemical and Biophysical Research Communications, 1982
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Tertiary structural differences between microbial serine proteases and pancreatic serine enzymesNature, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Studies on the Bacillus subtilis neutral-protease- and Bacillus thermoproteolyticus thermolysin-catalyzed hydrolysis of dipeptide substratesBiochemistry, 1970