Continuous monitoring of cerebrovascular autoregulation using functional ultrasound imaging in the piglet brain

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Abstract
Continuous real-time assessment of cerebral blood flow (CBF) and cerebrovascular autoregulation (CA) remains a major unmet clinical need in acute brain injury. Methods such as laser Doppler flowmetry (LDF), transcranial Doppler, or indirect indices lack accuracy and robustness. Functional ultrasound (fUS) is an emerging modality combining high spatiotemporal resolution, large field-of-view, and sensitivity to blood velocity and volume, making it a promising neuromonitoring tool. Piglets were equipped with arterial blood pressure (ABP), intracranial pressure (ICP), and LDF probes, plus cranial windows for fUS and red blood cell (RBC) flux imaging. CA was challenged by non-pharmacological ABP manipulation via intraaortic or intracaval balloon inflation. fUS hemodynamic parameters were compared with other modaliters across a CPP range of 10–150 mmHg. fUS provided continuous, stable intensity- and velocity-derived parameters across vessels types. CBF estimates correlated strongly with RBC flux and showed reproducibility comparable to LDF, with lower inter-animal variability. Autoregulation breakpoints were reliably identified by fUS, particularly the lower limit, while the upper limit was more variable. Parcellation confirmed robustness of fUS across brain regions. fUS images CBF and CA with higher stability and reproducibility than standard approaches, supporting its applicability for bedside neuromonitoring and clinical translation.
Funding Information
  • Fonds Wetenschappelijk Onderzoek - BE (G079623N)
  • Fonds Wetenschappelijk Onderzoek - BE (G091719N)
  • Fonds Wetenschappelijk Onderzoek - BE (1197818N)
  • Fonds Wetenschappelijk Onderzoek - BE (G0A4F24N)
  • Fonds Wetenschappelijk Onderzoek - BE (G055124N)
  • Fonds Wetenschappelijk Onderzoek - BE (12D7523N)
  • ERANET (964215 - UnscrAMBLY)
  • HORIZON-MSCA (101119916-SOPRANI)
  • Johnson & Johnson Research Chair (Profound analysis of cerebrovascular pressure autoregulation and associated physiological variables in severe traumatic brain injury)

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